Next Generation Sequencing (NGS) in Forensic Genetics and Anthropology Training Course

Introduction to Next-Generation Sequencing and Forensic Applications

• Overview of contemporary DNA sequencing methodologies
• Benefits of NGS compared to conventional forensic analytical methods
• Standard forensic applications of NGS technology

Fundamental Principles of Forensic Genetics

• DNA markers utilized in forensic examination
• Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs)
• Mitochondrial DNA and Y-chromosome analysis

NGS Workflows in Forensic Investigations

• Sample preparation and library construction
• Sequencing protocols for forensic samples
• Quality assurance and contamination prevention

Bioinformatics and NGS Data Analysis

• NGS data processing and alignment
• Variant calling and forensic interpretation
• Software tools for forensic genomic analysis

Forensic Anthropology and Ancestry Determination

• Genetic markers for ancestry and population studies
• NGS applications in ancient and degraded DNA analysis
• Case studies in forensic anthropology

NGS for Challenging and Degraded Samples

• Techniques for low-quality and low-quantity DNA
• Dealing with environmental and mixed DNA samples
• NGS applications in missing persons and disaster victim identification

Legal, Ethical, and Regulatory Considerations

• Privacy concerns in forensic genomics
• Regulatory frameworks for forensic DNA databases
• Ethical challenges in forensic genetic analysis

Future Trends in Forensic Genomics

• Advancements in sequencing technology
• Integration of AI in forensic genetics
• Emerging forensic applications of NGS

Summary and Next Steps